About the testing
Laboratory diagnostic procedures for chlamydial infections are demanding due to the peculiarities of the cell cycle and the intracellular deposition of chlamydia. The problems in obtaining samples and the inaccessibility of culturing and non-culturing methods therefore make serology the method of choice. Detection of chlamydial infections is based on the determination of antibody titers by the metod ELISA.
For testing the detection of anti-chlamydial antibodies of three isotypes IgM, IgG and IgA against specific antigens, we provide the ELISA-VIDITEST and MONO-VIDITEST kits from our own research and production.
ELISA-VIDITEST uses 96-well microtiter plates, while MONO-VIDITEST uses cassette 8-well format. All testing and evaluation takes place automatically in combination with our VIDIMAT analyser for both tests simultaneously and under the same incubation conditions.
Determination of specific antibodies
Chlamydia trachomatis and
- - ELISA-VIDITEST
- - MONO-VIDITEST
About the virus
It is one of the gram-negative (G-) bacteria with an incomplete bacterial wall. Chlamydia are energetic intracellular parasites because they do not have their own system for producing ATP and therefore obtain energy from the host living cell.
Elemental bodies (EB), which are infectious, metabolically inactive, enter the host cell by phagocytosis. Then it changes in the phagosome into non-infectious metabolically active reticular bodies (RB). During the next 24 - 48 hours, they divide intensively and then condense again in EB. After rupture, EB cells adhere to another host cell, infect it, and begin another reproduction cycle.
Chlamydia pneumoniae is the primary pathogen of the respiratory tract in the human population. Of all the types of chlamydia, it is the most common, the most dangerous, and the most infectious.
Chlamydia trachomatis is an obligate intracellular pathogen and one of the most common causes of sexually transmitted diseases.
About the infection
Primary infection of Ch. trachomatis / Ch. pneumoniae is characterized by a predominance of IgM antibodies within 2-4 weeks after infection. After 6-8 weeks, specific IgG and IgA antibodies begin to form. After acute infection with Ch.trachomatis / Ch.pneumoniae, usually after 2 - 6 months, IgM antibodies cannot be detected. The level of IgG antibodies decreases very slowly, while the level of IgA antibodies decreases faster. In primary chlamydial infections, the detection of IgM antibodies is an important diagnostic feature, but in the case of recurrent or chronic infections, the level of IgM antibodies is very low or non-existent. A negative finding of specific IgM antibodies in these cases does not exclude acute or recent infection.
Chlamydia pneumoniae causes infections of the respiratory tract. It is transmitted in a droplet manner.
Chlamydia trachomatis causes infections of the urogenital tract. It is one of the most common sexually transmitted agents. It causes various types of chronic inflammation. It is transmitted mainly through unprotected sexual intercourse.